PinPoint Xa-2
At a Glance
<protect>
| Type |
Expression |
|---|---|
| Origin of Replication | |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
T7 Promoter |
| Accession Number | |
| edit table |
</protect>
Notes
The PinPoint Xa Protein Purification System is used for the production and purification of fusion proteins that are biotinylated in vivo. The PinPoint Vectors an encoded endoproteinase Factor Xa proteolytic site that provides a way to separate the purification tag from the native protein. These vectors also carry a multiple cloning region for use in the construction of fusion proteins. The PinPoint Xa Vector contains the chloramphenicol acetyltransferase gene and is provided as a means of monitoring protein expression, purification and processing conditions.
The PinPoint™ Xa-2 Vector sequence is identical to PinPoint™ Xa-1 Vector, except for one additional adenosine at position 394.
History
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Sources
Promega. Discontinued.
References
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Sequence
1 ATGAAACTGA AGGTAACAGT CAACGGCACT GCGTATGACG TTGACGTTGA 51 CGTCGACAAG TCACACGAAA ACCCGATGGG CACCATCCTG TTCGGCGGCG 101 GCACCGGCGG CGCGCCGGCA CCGGCAGCAG GTGGCGCAGG CGCCGGTAAG 151 GCCGGAGAGG GCGAGATTCC CGCTCCGCTG GCCGGCACCG TCTCCAAGAT 201 CCTCGTGAAG GAGGGTGACA CGGTCAAGGC TGGTCAGACC GTGCTCGTTC 251 TCGAGGCCAT GAAGATGGAG ACCGAGATCA ACGCTCCCAC CGACGGCAAG 301 GTCGAGAAGG TCCTGGTCAA GGAGCGTGAC GCGGTGCAGG GCGGTCAGGG 351 TCTCATCAAG ATCGGGGATC TCGAGCTCAT CGAAGGTCGC GAAAGCTTCA 401 GCTGGGATCC GGTACCGATA TCAGATCTCC CGGGGCGGCC GCGATCTGGT 451 TCTATAGTGT CACCTAAATC GTATGTGTAT GATACATAAG GTTATGTATT 501 AATTGTAGCC GCGTTCTAAC GACAATATGT CCATATGGTG CACTCTCAGT 551 ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC 601 ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA 651 GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG 701 TCATCACCGA AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT 751 ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT 801 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT 851 TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA 901 ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA 951 TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG 1001 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA 1051 CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG 1101 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG 1151 GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA 1201 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC 1251 TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG 1301 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA 1351 GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG 1401 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC 1451 ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG 1501 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG 1551 CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG 1601 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT 1651 TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA 1701 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG 1751 ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC 1801 ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT 1851 AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG 1901 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC 1951 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC 2001 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT 2051 TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT 2101 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC 2151 CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC 2201 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA 2251 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG 2301 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA 2351 AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG 2401 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT 2451 GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA 2501 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA 2551 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT 2601 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT 2651 GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC 2701 AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG 2751 CGCGTTGGCC GATTCATTAA TGCAGGTTAA CCTGGCTTAT CGAAATTAAT 2801 ACGACTCACT ATAGGGAGAC CGGCCTCGAG CAGCAAGGAG ATGGCGCCCA 2851 ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG 2901 CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC 2951 GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA 3001 TGCGTCCGGC GTAGAGGATC GATCCGGGCT TATCGACTGC ACGGTGCACC 3051 AATGCTTCTG GCGTCAGGCA GCCATCGGAA GCTGTGGTAT GGCTGTGCAG 3101 GTCGTAAATC ACTGCATAAT TCGTGTCGCT CAAGGCGCAC TCCCGTTCTG 3151 GATAATGTTT TTTGCGCCGA CATCATAACG GTTCTGGCAA ATATTCTGAA 3201 ATGAGCTGTT GACAATTAAT CATCGGCTCG TATAATGTGT GGAATTGTGA 3251 GCGGATAACA ATTTCACACA GGAAACAGAA TTCCCAGCTT GGCTGCAGAA 3301 CCATTCCATT CGTTGATCCG GGAGTAACTC AC
