PinPoint Xa-1
At a Glance
<protect>
| Type |
Expression |
|---|---|
| Origin of Replication | |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
T7 Promoter |
| Accession Number | |
| edit table |
</protect>
Notes
PinPoint Vectors include encoded endoproteinase Factor Xa proteolytic site (provides a way to separate purification tag from native protein) and a multiple cloning region for ease in the making of fusion proteins.
History
This vector was made for use with the PinPoint™ Xa Protein Purification System(a,b,c). A Promega product designed for the production and purification of fusion proteins which are biotinylated in vivo.
Sources
References
See Help:References for how to manage references in EcoliWiki.
Sequence
1 atgaaactga aggtaacagt caacggcact gcgtatgacg ttgacgttga cgtcgacaag
61 tcacacgaaa acccgatggg caccatcctg ttcggcggcg gcaccggcgg cgcgccggca
121 ccggcagcag gtggcgcagg cgccggtaag gccggagagg gcgagattcc cgctccgctg
181 gccggcaccg tctccaagat cctcgtgaag gagggtgaca cggtcaaggc tggtcagacc
241 gtgctcgttc tcgaggccat gaagatggag accgagatca acgctcccac cgacggcaag
301 gtcgagaagg tcctggtcaa ggagcgtgac gcggtgcagg gcggtcaggg tctcatcaag
361 atcggggatc tcgagctcat cgaaggtcgc gaagcttcag ctgggatccg gtaccgatat
421 cagatctccc ggggcggccg cgatctggtt ctatagtgtc acctaaatcg tatgtgtatg
481 atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgtc catatggtgc
541 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
601 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
661 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
721 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
781 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
841 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
901 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
961 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
1021 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
1081 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
1141 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
1201 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
1261 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
1321 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
1381 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
1441 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
1501 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
1561 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
1621 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
1681 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
1741 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
1801 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
1861 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
1921 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
1981 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
2041 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
2101 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
2161 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
2221 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
2281 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
2341 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
2401 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
2461 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
2521 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
2581 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
2641 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
2701 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
2761 attcattaat gcaggttaac ctggcttatc gaaattaata cgactcacta tagggagacc
2821 ggcctcgagc agcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat
2881 acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt
2941 gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat
3001 gcgtccggcg tagaggatcg atccgggctt atcgactgca cggtgcacca atgcttctgg
3061 cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg tcgtaaatca ctgcataatt
3121 cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt ttgcgccgac atcataacgg
3181 ttctggcaaa tattctgaaa tgagctgttg acaattaatc atcggctcgt ataatgtgtg
3241 gaattgtgag cggataacaa tttcacacag gaaacagaat tcccagcttg gctgcagaac
3301 cattccattc gttgatccgg gagtaactca c
