PinPoint Control
At a Glance
<protect>
| Type |
Expression |
|---|---|
| Origin of Replication | |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
T7 promoter |
| Accession Number |
PinPoint™ Xa-1, U47626 |
| edit table |
</protect>
Notes
The PinPoint™ Xa Protein Purification System is used for the production and purification of fusion proteins that are biotinylated in vivo. The PinPoint Vectors an encoded endoproteinase Factor Xa (pronounced “ten a”) proteolytic site that provides a way to separate the purification tag from the native protein. These vectors also carry a multiple cloning region for use in the construction of fusion proteins. The PinPoint Xa Vector contains the chloramphenicol acetyltransferase gene and is provided as a means of monitoring protein expression, purification and processing conditions.
History
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Sources
References
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Sequence
1 ATGAAACTGA AGGTAACAGT CAACGGCACT GCGTATGACG TTGACGTTGA 51 CGTCGACAAG TCACACGAAA ACCCGATGGG CACCATCCTG TTCGGCGGCG 101 GCACCGGCGG CGCGCCGGCA CCGGCAGCAG GTGGCGCAGG CGCCGGTAAG 151 GCCGGAGAGG GCGAGATTCC CGCTCCGCTG GCCGGCACCG TCTCCAAGAT 201 CCTCGTGAAG GAGGGTGACA CGGTCAAGGC TGGTCAGACC GTGCTCGTTC 251 TCGAGGCCAT GAAGATGGAG ACCGAGATCA ACGCTCCCAC CGACGGCAAG 301 GTCGAGAAGG TCCTGGTCAA GGAGCGTGAC GCGGTGCAGG GCGGTCAGGG 351 TCTCATCAAG ATCGGGGATC TCGAGCTCAT CGAAGGTCGC GAAGCTTCAG 401 CTGGGATCCT AGCTATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT 451 ATATCCCAAT GGCATCGTAA AGAACATTTT GAGGCATTTC AGTCAGTTGC 501 TCAATGTACC TATAACCAGA CCGTTCAGCT GGATATTACG GCCTTTTTAA 551 AGACCGTAAA GAAAAATAAG CACAAGTTTT ATCCGGCCTT TATTCACATT 601 CTTGCCCGCC TGATGAATGC TCATCCGGAA TTCCGTATGG CAATGAAAGA 651 CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC 701 ATGAGCAAAC TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT 751 TTCCGGCAGT TTCTACACAT ATATTCGCAA GATGTGGCGT GTTACGGTGA 801 AAACCTGGCC TATTTCCCTA AAGGGTTTAT TGAGAATATG TTTTTCGTCT 851 CAGCCAATCC CTGGGTGAGT TTCACCAGTT TTGATTTAAA CGTGGCCAAT 901 ATGGACAACT TCTTCGCCCC CGTTTTCACC ATGGGCAAAT ATTATACGCA 951 AGGCGACAAG GTGCTGATGC CGCTGGCGAT TCAGGTTCAT CATGCCGTTT 1001 GTGATGGCTT CCATGTCGGC AGAATGCTTA ATGAATTACA ACAGTACTGC 1051 GATGAGTGGC AGGGCGGGGC GTAAGAAGCT GGGCGGCCGC GATCTGGTTC 1101 TATAGTGTCA CCTAAATCGT ATGTGTATGA TACATAAGGT TATGTATTAA 1151 TTGTAGCCGC GTTCTAACGA CAATATGTCC ATATGGTGCA CTCTCAGTAC 1201 AATCTGCTCT GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC 1251 CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA 1301 CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC 1351 ATCACCGAAA CGCGCGAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT 1401 AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT 1451 CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC 1501 AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT 1551 ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT 1601 CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT 1651 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA 1701 TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA 1751 GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT 1801 ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT 1851 ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT 1901 ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG 1951 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG 2001 AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT 2051 CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC 2101 CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG 2151 AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG 2201 GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT 2251 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG 2301 CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG 2351 ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT 2401 AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT 2451 ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG 2501 GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT 2551 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT 2601 GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA 2651 CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 2701 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC 2751 TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT 2801 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 2851 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG 2901 CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG 2951 CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC ATTGAGAAAG 3001 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA 3051 GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG 3101 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 3151 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG 3201 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC 3251 TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA 3301 GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG 3351 TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG 3401 CGTTGGCCGA TTCATTAATG CAGGTTAACC TGGCTTATCG AAATTAATAC 3451 GACTCACTAT AGGGAGACCG GCCTCGAGCA GCAAGGAGAT GGCGCCCAAC 3501 AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT 3551 CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA 3601 TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG 3651 CGTCCGGCGT AGAGGATCGA TCCGGGCTTA TCGACTGCAC GGTGCACCAA 3701 TGCTTCTGGC GTCAGGCAGC CATCGGAAGC TGTGGTATGG CTGTGCAGGT 3751 CGTAAATCAC TGCATAATTC GTGTCGCTCA AGGCGCACTC CCGTTCTGGA 3801 TAATGTTTTT TGCGCCGACA TCATAACGGT TCTGGCAAAT ATTCTGAAAT 3851 GAGCTGTTGA CAATTAATCA TCGGCTCGTA TAATGTGTGG AATTGTGCGC 3901 GGATAACAAT TTCACACAGG AAACAGAATT CCCAGCTTGG CTGCAGAACC 3951 ATTCCATTCG TTGATCCGGG AGTAACTCAC
