Phage T4
Bacteriophage T4 is a member of the ICTV family myoviridae, long-tailed viruses with contractile tails. T4 is the namesake of the T4-like virus genus. T4 and its relatives were used for many of the key experiments in the history of molecular biology.
Contents
Morphology
Genome Organization
T4 has a circular genetic map[1], but the T4 genome is physically packaged as linear dsDNA. Headful packaging incorporates DNA that is circularly permuted[2] and terminally redundant[3] with respect to the genetic map.
Life cycle
Lytic cycle
Adsorbtion and Injection
Early gene expression
Middle gene expression
DNA replication
Initiation of DNA replication has been reviewed by Kreuzer and Brister[4] and many references needed. Replication starts early in infection from multiple origins and then shifts to a recombination-dependent phase.
T4 replisomes have been intensely studied, and work from the Alberts lab led to the "trombone model" for coupling of leading and lagging strand progression. High resolution structures for part or all of several replisome proteins have been determined (reviewed by Mueser et al. [5])
Late gene expression
Packaging
Lysis
Other phenomena of interest
intron homing
base modification
lysis inhibition
exclusion
See also
- List of T4 gene pages in EcoliWiki
- GGC T4-like Genome website!
- Computer animation of T4 infection
- Phage T4 Taxonomy on NCBI
- International Committee on Taxonomy of Viruses
- NC 000866.4 on NCBI
- GenBank record for T4
- T4 in Wikipedia
References
See Help:References for how to manage references in EcoliWiki.
- ↑ STREISINGER, G et al. (1964) CHROMOSOME STRUCTURE IN PHAGE T4. I. CIRCULARITY OF THE LINKAGE MAP. Proc. Natl. Acad. Sci. U.S.A. 51 775-9 PubMed
- ↑ THOMAS, CA Jr & RUBENSTEIN, I (1964) THE ARRANGEMENTS OF NUCLEOTIDE SEQUENCES IN T2 AND T5 BACTERIOPHAGE DNA MOLECULES. Biophys. J. 4 93-106 PubMed
- ↑ Séchaud, J et al. (1965) Chromosome structure in phage T4, II. Terminal redundancy and heterozygosis. Proc. Natl. Acad. Sci. U.S.A. 54 1333-9 PubMed
- ↑ Kreuzer, KN & Brister, JR (2010) Initiation of bacteriophage T4 DNA replication and replication fork dynamics: a review in the Virology Journal series on bacteriophage T4 and its relatives. Virol. J. 7 358 PubMed
- ↑ Mueser, TC et al. (2010) Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives. Virol. J. 7 359 PubMed
