PMID: 6780756
| Citation |
Albrechtsen, H and Ahmad, SI (1980) Regulation of the synthesis of nucleoside catabolic enzymes in Escherichia coli: further analysis of a deo Oc mutant strain. Mol. Gen. Genet. 179:457-60 |
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| Abstract |
Four genes, deoA, deoB, deoC, and deoD, involved in the synthesis of nucleoside and deoxynucleoside catabolic enzymes, are located contiguously in the order C-A-B-D on the linkage map of E. coli. They constitute two overlapping operons, one transcribing all the four genes and the other deoB and deoD. To the left of deoC are located two promoter-operator regions in the order deoPO-cytPO. They are involved in controlling the expression of the tetracistronic mRNA. For efficient binding of RNA polymerase at the cytPO site the cAMP+CRP complex is required, whereas binding of RNA polymerase at the deoPO site is independent of this complex. Evidence is available for the existence of yet another controlling site, PO-3, located between deoA and deoB; this controls the expression of deoB and deoD. Both the operons are transcribed in a clockwise direction. An operator constitutive (Oc) type mutant affecting the synthesis of all four deo enzymes has been analysed. Because of this mutation the strain has become insensitive to catabolite repression. The results confirm the order of the gene in the controlling region to be deoPO-cytPO and the mutation, previously analysed as a deletion, appears to have deleted cytPO deoC region of the chromosome. |
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| Keywords |
Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Escherichia coli/genetics; Gene Expression Regulation; Genes, Regulator; Mutation; Operon; Pentosyltransferases/genetics; Purine-Nucleoside Phosphorylase/biosynthesis; Purine-Nucleoside Phosphorylase/genetics |
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