PMID: 3053647
| Citation |
Aldea, M, Hernández-Chico, C, de la Campa, AG, Kushner, SR and Vicente, M (1988) Identification, cloning, and expression of bolA, an ftsZ-dependent morphogene of Escherichia coli. J. Bacteriol. 170:5169-76 |
|---|---|
| Abstract |
A newly found morphogene of Escherichia coli, bolA, mapping at min 10 of the genetic map, was cloned in a 7.2-kilobase BamHI fragment and identified by its ability to produce osmotically stable spherical cells when overexpressed. This gene codes for a polypeptide of 13 kilodaltons. Overexpression of bolA+ was achieved in low-copy-number vectors with operon fusions to the tet and lac promoters, indicating a clockwise direction of transcription. While no modification of any of the penicillin-binding proteins was observed, morphological effects due to overexpression of bolA+ were shown to be dependent on the presence of an active ftsZ gene product. Our results suggest the existence of a mechanism mediated by FtsZ for modifying the conformation of nascent murein in the early steps of septum formation. |
| Links | |
| Keywords |
Cell Division; Cloning, Molecular; Escherichia coli/cytology; Escherichia coli/genetics; Escherichia coli/growth & development; Genes, Bacterial; Genetic Linkage; Genetic Vectors; Morphogenesis; Plasmids; Promoter Regions, Genetic; Restriction Mapping; Transcription, Genetic; Transduction, Genetic |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.