PMID:9933617
| Citation |
Benov, L and Fridovich, I (1999) Why superoxide imposes an aromatic amino acid auxotrophy on Escherichia coli. The transketolase connection. J. Biol. Chem. 274:4202-6 |
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| Abstract |
The lack of superoxide dismutase and the consequent elevation of [O2-] imposes, on Escherichia coli, auxotrophies for branched chain, sulfur-containing, and aromatic amino acids. The former two classes of auxotrophies have already been explained, whereas the third is explained herein. Thus O2- is shown to interfere with the production of erythrose-4-phosphate, which is essential for the first step of the aromatic biosynthetic pathway. It does so by oxidizing the 1, 2-dihydroxyethyl thiamine pyrophosphate intermediate of transketolase and inactivating this enzyme. |
| Links | |
| Keywords |
Bacterial Proteins/physiology; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/metabolism; Oxygen/metabolism; Paraquat/pharmacology; Shikimic Acid/pharmacology; Sugar Phosphates/metabolism; Superoxide Dismutase/physiology; Superoxides/metabolism; Transketolase/metabolism |
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