PMID:9922273
| Citation |
Park, Y and Park, C (1999) Topology of RbsC, a membrane component of the ribose transporter, belonging to the AraH superfamily. J. Bacteriol. 181:1039-42 |
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| Abstract |
RbsC of Escherichia coli is the hydrophobic membrane component of ribose uptake system classified as the ATP-binding cassette transporter. To understand the structure and function of RbsC, its transmembrane topology was investigated by using 64 RbsC-PhoA fusions isolated either specifically or randomly. In order to confirm the cytoplasmic location of the short C-terminal region (5 amino acids), inside-out or right-side-out membrane vesicles were generated, and the C-terminal region was found to be digested by carboxypeptidase A only in inside-out vesicles. This result is consistent with the model, based on the results of alkaline phosphatase fusions, in which the protein traverses the membrane six times and the N and C termini are exposed to the cytoplasm. |
| Links | |
| Keywords |
ATP-Binding Cassette Transporters/chemistry; ATP-Binding Cassette Transporters/genetics; Alkaline Phosphatase; Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Carboxypeptidases; Carboxypeptidases A; Cell Membrane/physiology; Cell Membrane/ultrastructure; Cyclin-Dependent Kinases/chemistry; Cytoplasm/physiology; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins; Models, Molecular; Molecular Sequence Data; Polymerase Chain Reaction; Protein Structure, Secondary; Recombinant Fusion Proteins/chemistry; Restriction Mapping |
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