PMID:9884223
| Citation |
Alonzo, S, Heyde, M, Laloi, P and Portalier, R (1998) Analysis of the effect exerted by extracellular pH on the maltose regulon in Escherichia coli K-12. Microbiology (Reading, Engl.) 144 ( Pt 12):3317-25 |
|---|---|
| Abstract |
The Escherichia coli maltose regulon consists of five operons under the control of the MalT transcriptional activator. lac operon fusions were constructed in vitro with the MalT-dependent promoter and with the malT promoter itself. beta-Galactosidase activity displayed by these fusions during growth at different external pH (pHo) revealed that growth at a pHo higher than 6 stimulates the transcription of malT- and MalT-controlled genes in the absence or presence of maltose. Using a malTp1 malTp10 promoter that is cAMP-CRP (cAMP receptor protein)-independent, it was demonstrated that CRP is essential for malT pHo regulation and that the pHo-dependent activity of malKp is a direct consequence of malT regulation. The pHo regulation displayed by a deleted but still functional malT promoter fused to lacZ demonstrates that this minimal promoter contains all the regulatory regions for establishing pHo regulation. In the absence of MIc, a repressor of malT expression, the pHo regulation of malT was still effective. It is proposed that binding of cAMP-CRP at malTp may be affected by malTp topology induced by pHo or that a pHo-dependent effector may act in concert with the cAMP-CRP complex. |
| Links | |
| Keywords |
ATP-Binding Cassette Transporters/genetics; Bacterial Proteins/genetics; Bacterial Proteins/physiology; Carrier Proteins; Cyclic AMP Receptor Protein/physiology; DNA-Binding Proteins; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Hydrogen-Ion Concentration; Maltose/physiology; Regulon; Transcription Factors/genetics; Transcription Factors/physiology |
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