PMID:9875851
| Citation |
Anderson, DG and Kowalczykowski, SC (1998) Reconstitution of an SOS response pathway: derepression of transcription in response to DNA breaks. Cell 95:975-9 |
|---|---|
| Abstract |
E. coli responds to DNA damage by derepressing the transcription of about 20 genes that make up the SOS pathway. Genetic analyses have shown that SOS induction in response to double-stranded DNA (dsDNA) breaks requires LexA repressor, and the RecA and RecBCD enzymes--proteins best known for their role as initiators of dsDNA break repair and homologous recombination. Here we demonstrate that purified RecA protein, RecBCD enzyme, single-stranded DNA-binding (SSB) protein, and LexA repressor respond to dsDNA breaks in vitro by derepressing transcription from an SOS promoter. Interestingly, derepression is more rapid if the DNA containing the dsDNA break has a chi recombination hot spot (5'-GCTGGTGG-3'), suggesting a novel regulatory role for one of the most overrepresented octamers in the E. coli genome. |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Chromosome Breakage/physiology; DNA Damage/physiology; DNA Repair/genetics; DNA-Binding Proteins/metabolism; Deoxyribonucleases, Type II Site-Specific/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/metabolism; Exodeoxyribonuclease V; Exodeoxyribonucleases/metabolism; Gene Expression Regulation, Bacterial/drug effects; Models, Genetic; Molecular Sequence Data; Promoter Regions, Genetic/genetics; Rec A Recombinases/genetics; Rec A Recombinases/metabolism; Recombination, Genetic/genetics; Repressor Proteins/genetics; Repressor Proteins/metabolism; SOS Response (Genetics)/genetics; Serine Endopeptidases/genetics; Serine Endopeptidases/metabolism; Transcription, Genetic/genetics |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.