PMID:9871110
| Citation |
Kiser, KB and Schmidt, MG (1999) Regulation of the Escherichia coli secA gene is mediated by two distinct RNA structural conformations. Curr. Microbiol. 38:113-21 |
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| Abstract |
Expression from the secA gene, encoding a key component of the general secretory pathway of Escherichia coli, is influenced by the secretion status of the cell, autogenous translational repression, and translational coupling to the upstream gene, X. SecA binds to its mRNA in a region overlapping its ribosome binding site, thus competing with ribosomes that would initiate secA translation. Mapping of the geneX-secA mRNA secondary structure has demonstrated that the RNA can adopt two distinct conformations in solution. The first conformation arises from the base-pairing of the secA Shine-Dalgarno (SD) sequence with the geneX terminus. The second conformation, in which the secA SD sequence is no longer paired with the geneX terminus, contains a GC-rich stem upstream of the secA SD sequence. The presence of this GC-rich stem is supported by structure mapping of a mutant RNA containing a deletion in the geneX terminus. The former structure appears to be involved in translational coupling by directly linking the geneX and secA sequences, where geneX translation activates secA translational initiation through the unpairing and unmasking of the secA SD sequence. As indicated by SecA-RNA binding assays, the latter structure is probably involved in SecA binding and translational repression of the secA gene. The stabilizing effect of magnesium ions toward occlusion of the secA SD sequence supports the presence of RNA tertiary structure in this regulatory domain. |
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| Keywords |
Adenosine Triphosphatases/genetics; Bacterial Proteins/genetics; Base Sequence; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Magnesium/pharmacology; Membrane Transport Proteins; Molecular Sequence Data; Nucleic Acid Conformation; RNA, Messenger/chemistry; Ribonucleases/pharmacology |
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