PMID:9862472

Citation	Sbai, M and Alix, JH (1998) DnaK-dependent ribosome biogenesis in Escherichia coli: competition for dominance between the alleles dnaK756 and dnaK+. Mol. Gen. Genet. 260:199-206
Abstract	The Escherichia coli chaperone DnaK is vital for many cellular functions, including ribosome biogenesis at high temperature. Thus, the dnaK756-ts (lambdaR) mutant, at the non-permissive temperature, is inhibited at a late stage of ribosome assembly, yielding 21S, 32S and 45S precursor particles. This defect, unlike the lambda resistance and thermosensitivity phenotypes, is not complemented by lysogenisation with a transducing phage lambda dnaK+ bearing the wild-type dnaK gene. However this dominant phenotype becomes recessive when dnaK+ is expressed from a medium-copy-number plasmid. On the other hand, an excess of DnaK causes an unexpected dominant-lethal effect of the dnaK756 allele near non-permissive temperatures. This interplay between the dnaK+ and dnaK756 alleles supports the idea of that DnaK oligomers form in the cell.
Links	PubMed
Keywords	Alleles; Bacteriophage lambda/genetics; Escherichia coli/physiology; Escherichia coli/virology; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Genes, Dominant; Genes, Lethal; Genetic Complementation Test; HSP70 Heat-Shock Proteins/chemistry; HSP70 Heat-Shock Proteins/genetics; HSP70 Heat-Shock Proteins/metabolism; Phenotype; Plasmids; Protein Folding; Ribosomes/metabolism
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