PMID:9860956
| Citation |
Zylicz, M, Liberek, K, Wawrzynow, A and Georgopoulos, C (1998) Formation of the preprimosome protects lambda O from RNA transcription-dependent proteolysis by ClpP/ClpX. Proc. Natl. Acad. Sci. U.S.A. 95:15259-63 |
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| Abstract |
Using the bacteriophage lambda DNA replication system, composed entirely of purified proteins, we have tested the accessibility of the short-lived lambda O protein to the ClpP/ClpX protease during the various stages of lambda DNA replication. We find that binding of lambda O protein to its orilambda DNA sequence, leading to the so-called "O-some" formation, largely inhibits its degradation. On the contrary, under conditions permissive for transcription, the lambda O protein bound to the orilambda sequence becomes largely accessible to ClpP/ClpX-mediated proteolysis. However, when the lambda O protein is part of the larger orilambda:O.P.DnaB preprimosomal complex, transcription does not significantly increase ClpP/ClpX-dependent lambda O degradation. These results show that transcription can stimulate proteolysis of a protein that is required for the initiation of DNA replication. |
| Links | |
| Keywords |
Adenosine Triphosphatases/metabolism; Bacterial Proteins; Bacteriophage lambda/genetics; Bacteriophage lambda/physiology; DNA Helicases/metabolism; DNA Replication; DNA, Viral/genetics; DNA, Viral/metabolism; DNA-Directed RNA Polymerases/metabolism; DnaB Helicases; Endopeptidase Clp; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli/virology; Kinetics; Models, Genetic; Replication Origin; Serine Endopeptidases/metabolism; Transcription, Genetic; Viral Proteins/metabolism; Virus Replication |
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