PMID:9852024

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Citation

Ogawa, W, Kim, YM, Mizushima, T and Tsuchiya, T (1998) Cloning and expression of the gene for the Na+-coupled serine transporter from Escherichia coli and characteristics of the transporter. J. Bacteriol. 180:6749-52

Abstract

We cloned a gene (sstT) for the Na+/serine symporter from the chromosome of Escherichia coli by using a low-copy-number vector and sequenced it. According to the deduced amino acid sequence, the transporter (SstT) consists of 414 amino acid residues. Hydropathy analysis suggested that the SstT protein possesses 9, instead of 12, hydrophobic domains.

Links

PubMed PMC107783

Keywords

Amino Acid Transport Systems; Amino Acid Transport Systems, Basic; Amino Acid Transport Systems, Neutral; Biological Transport; Carrier Proteins/genetics; Carrier Proteins/metabolism; Chromosome Mapping; Cloning, Molecular; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Gene Expression Regulation, Bacterial/drug effects; Genes, Bacterial; Sequence Analysis, DNA; Serine/metabolism; Sodium/metabolism; Tryptophan/metabolism; Tryptophan/pharmacology

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