PMID:9829961

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Citation

Boyle, DS and Donachie, WD (1998) mraY is an essential gene for cell growth in Escherichia coli. J. Bacteriol. 180:6429-32

Abstract

The synthesis of the murein precursor lipid I is performed by MraY. We have shown that mraY is an essential gene for cell growth. Cells depleted of MraY first swell and then lyse. The expression of mraY DNA in vitro produces a 40-kDa polypeptide detectable by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Links

PubMed PMC107738

Keywords

Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Base Sequence; Cell Division/genetics; DNA Primers/genetics; DNA, Bacterial/genetics; Escherichia coli/cytology; Escherichia coli/genetics; Escherichia coli/growth & development; Gene Expression; Genes, Bacterial; Genetic Complementation Test; Mutagenesis, Insertional; Phenotype; Plasmids/genetics; Transferases

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