PMID:9811673
| Citation |
Bohn, C and Bouloc, P (1998) The Escherichia coli cmlA gene encodes the multidrug efflux pump Cmr/MdfA and is responsible for isopropyl-beta-D-thiogalactopyranoside exclusion and spectinomycin sensitivity. J. Bacteriol. 180:6072-5 |
|---|---|
| Abstract |
Expression of cloned genes from isopropyl-beta-D-thiogalactopyranoside (IPTG)-regulated promoters is lowered when the Escherichia coli CmlA/Cmr/MdfA efflux pump is overexpressed, probably due to IPTG exclusion from the cytoplasm. The previously reported cmlA1 mutation confers a similar phenotype. cmlA1 contains an IS30 insertion upstream of cmr/mdfA, which creates a putative promoter. CmlA overproduction also causes spectinomycin hypersensitivity. |
| Links | |
| Keywords |
Alleles; Bacterial Proteins; Carrier Proteins/genetics; Carrier Proteins/metabolism; Drug Resistance, Microbial/genetics; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression; Isopropyl Thiogalactoside/metabolism; Membrane Proteins/genetics; Membrane Proteins/metabolism; Membrane Transport Proteins; Mutagenesis; Plasmids; Spectinomycin/metabolism; beta-Galactosidase/biosynthesis |
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