PMID:9811641
| Citation |
Boogerd, FC, Boe, L, Michelsen, O and Jensen, PR (1998) atp Mutants of Escherichia coli fail to grow on succinate due to a transport deficiency. J. Bacteriol. 180:5855-9 |
|---|---|
| Abstract |
Escherichia coli atp mutants, which lack a functional H+-ATPase complex, are capable of growth on glucose but not on succinate or other C4-dicarboxylates (Suc- phenotype). Suc+ revertants of an atp deletion strain were isolated which were capable of growth on succinate even though they lack the entire H+-ATPase complex. Complementation in trans with the yhiF gene suppressed the growth of the Suc+ mutants on succinate, which implicates the yhiF gene product in the regulation of C4-dicarboxylate metabolism. Indeed, when the E. coli C4-dicarboxylate transporter (encoded by the dctA gene) was expressed in trans, the Suc- phenotype of the atp deletion strain reverted to Suc+, which shows that the reason why the E. coli atp mutant is unable to grow aerobically on C4-dicarboxylates is insufficient transport capacity for these substrates. |
| Links | |
| Keywords |
Bacterial Proteins; Biological Transport; Carrier Proteins/genetics; Chromosome Mapping; Culture Media; Dicarboxylic Acid Transporters; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Gene Deletion; Genetic Complementation Test; Mutagenesis; Phenotype; Proton-Translocating ATPases/genetics; Proton-Translocating ATPases/metabolism; Succinates/metabolism; Transposases |
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