PMID:9774546
| Citation |
Boekema, EJ, Stuart, M, Koning, RI, Keegstra, W, Brisson, A, Verheij, HM and Dekker, N (1998) A 7.4-A projection structure of outer membrane phospholipase A from Escherichia coli by electron crystallography. J. Struct. Biol. 123:67-71 |
|---|---|
| Abstract |
Outer membrane phospholipase A (OMPLA) is one of the few enzymes present in the outer membrane of Escherichia coli. Two-dimensional crystals of OMPLA were grown by reconstitution of purified protein into lipid bilayers via detergent dialysis and were studied by electron crystallography. A 7.4-A projection map reveals OMPLA molecules exhibiting an oval-shaped domain of 30 x 20 A resembling the beta-barrel structure characteristic of porins, which is associated with a 25-A elongated domain of lower density. |
| Links |
PubMed Online version:10.1006/jsbi.1998.4013 |
| Keywords |
Bacterial Outer Membrane Proteins/ultrastructure; Crystallization; Escherichia coli/enzymology; Lipid Bilayers/metabolism; Microscopy, Electron; Phospholipases A/ultrastructure; Porins/chemistry; Protein Structure, Secondary |
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