PMID:9774349
| Citation |
Zhang, A, Altuvia, S, Tiwari, A, Argaman, L, Hengge-Aronis, R and Storz, G (1998) The OxyS regulatory RNA represses rpoS translation and binds the Hfq (HF-I) protein. EMBO J. 17:6061-8 |
|---|---|
| Abstract |
The OxyS regulatory RNA integrates the adaptive response to hydrogen peroxide with other cellular stress responses and protects against DNA damage. Among the OxyS targets is the rpoS-encoded sigma(s) subunit of RNA polymerase. Sigma(s) is a central regulator of genes induced by osmotic stress, starvation and entry into stationary phase. We examined the mechanism whereby OxyS represses rpoS expression and found that the OxyS RNA inhibits translation of the rpoS message. This repression is dependent on the hfq-encoded RNA-binding protein (also denoted host factor I, HF-I). Co-immunoprecipitation and gel mobility shift experiments revealed that the OxyS RNA binds Hfq, suggesting that OxyS represses rpoS translation by altering Hfq activity. |
| Links |
PubMed PMC1170932 Online version:10.1093/emboj/17.20.6061 |
| Keywords |
Bacterial Proteins/physiology; Base Sequence; Carrier Proteins/physiology; DNA-Binding Proteins; Escherichia coli; Escherichia coli Proteins; Host Factor 1 Protein; Integration Host Factors; Molecular Sequence Data; Nucleic Acid Conformation; Oxidative Stress/physiology; Precipitin Tests; RNA, Bacterial/physiology; RNA-Binding Proteins/physiology; Regulatory Sequences, Nucleic Acid/physiology; Repressor Proteins/physiology; Sigma Factor/physiology; Transcription Factors/physiology; Untranslated Regions/physiology |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.