PMID:9771897

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Citation

Yamada, H, Hanaki, N, Imamura, A, Ueguchi, C and Mizuno, T (1998) An Arabidopsis protein that interacts with the cytokinin-inducible response regulator, ARR4, implicated in the His-Asp phosphorylay signal transduction. FEBS Lett. 436:76-80

Abstract

Previously, Arabidopsis thaliana was shown to possess a set of response regulators (ARR-series), which are implicated in the prokaryotic type of signal transduction mechanism, generally referred to as the His-Asp phosphorylay. Among them, ARR4 is a typical phospho-accepting response regulator, whose expression was recently demonstrated to be rapidly induced by a cytokinin-treatment of the plant. To gain insight into the presumed His-Asp phosphotransfer signaling mechanism as well as the role of ARR4 in this higher plant, in this study we adopt the widely used yeast two-hybrid system, and report the identification of an Arabidopsis protein that has an ability to interact physically with the cytokinin-inducible ARR4 response regulator.

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Keywords

Amino Acid Sequence; Arabidopsis/chemistry; Arabidopsis/metabolism; Arabidopsis Proteins; Aspartic Acid/metabolism; Cloning, Molecular; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Histidine/metabolism; Hybrid Cells; Molecular Sequence Data; Phosphorylation; Plant Proteins/genetics; Plant Proteins/metabolism; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Sequence Homology, Amino Acid; Signal Transduction; Substrate Specificity; Yeasts/genetics

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