PMID:9770456

Citation	Li, Z, Pandit, S and Deutscher, MP (1998) Polyadenylation of stable RNA precursors in vivo. Proc. Natl. Acad. Sci. U.S.A. 95:12158-62
Abstract	Polyadenylation at the 3' terminus has long been considered a specific feature of mRNA and a few other unstable RNA species. Here we show that stable RNAs in Escherichia coli can be polyadenylated as well. RNA molecules with poly(A) tails are the major products that accumulate for essentially all stable RNA precursors when RNA maturation is slowed because of the absence of processing exoribonucleases; poly(A) tails vary from one to seven residues in length. The polyadenylation process depends on the presence of poly(A) polymerase I. A stochastic competition between the exoribonucleases and poly(A) polymerase is proposed to explain the accumulation of polyadenylated RNAs. These data indicate that polyadenylation is not unique to mRNA, and its widespread occurrence suggests that it serves a more general function in RNA metabolism.
Links	PubMed PMC22801
Keywords	Base Sequence; Cloning, Molecular; DNA Primers; Escherichia coli Proteins; Poly A/metabolism; Polynucleotide Adenylyltransferase/metabolism; RNA Precursors/metabolism; RNA, Ribosomal, 5S/metabolism; Reverse Transcriptase Polymerase Chain Reaction
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.