PMID:9765561
| Citation |
Shibayama, K, Ohsuka, S, Tanaka, T, Arakawa, Y and Ohta, M (1998) Conserved structural regions involved in the catalytic mechanism of Escherichia coli K-12 WaaO (RfaI). J. Bacteriol. 180:5313-8 |
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| Abstract |
Escherichia coli K-12 WaaO (formerly known as RfaI) is a nonprocessive alpha-1,3 glucosyltransferase, involved in the synthesis of the R core of lipopolysaccharide. By comparing the amino acid sequence of WaaO with those of 11 homologous alpha-glycosyltransferases, four strictly conserved regions, I, II, III, and IV, were identified. Since functionally related transferases are predicted to have a similar architecture in the catalytic sites, it is assumed that these four regions are directly involved in the formation of alpha-glycosidic linkage from alpha-linked nucleotide diphospho-sugar donor. Hydrophobic cluster analysis revealed a conserved domain at the N termini of these alpha-glycosyltransferases. This domain was similar to that previously reported for beta-glycosyltransferases. Thus, this domain is likely to be involved in the formation of beta-glycosidic linkage between the donor sugar and the enzyme at the first step of the reaction. Site-directed mutagenesis analysis of E. coli K-12 WaaO revealed four critical amino acid residues. |
| Links | |
| Keywords |
Amino Acid Sequence; Carbohydrate Sequence; Catalytic Domain; Cluster Analysis; Conserved Sequence; Escherichia coli/enzymology; Escherichia coli/genetics; Glucosyltransferases/genetics; Glucosyltransferases/metabolism; Lipopolysaccharides/biosynthesis; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Conformation; Sequence Analysis; Sequence Homology, Amino Acid |
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