PMID:9749677
| Citation |
Schembri, MA, Olsen, PB and Klemm, P (1998) Orientation-dependent enhancement by H-NS of the activity of the type 1 fimbrial phase switch promoter in Escherichia coli. Mol. Gen. Genet. 259:336-44 |
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| Abstract |
Phase variation of type 1 fimbriation in Escherichia coli is associated with the inversion of a 314-bp DNA element, positioned proximal to and upstream of fimA, which encodes the major type 1 fimbrial subunit. This DNA switch region contains a promoter that drives transcription of fimA only when the switch is in the ON orientation. Using chromosomal and plasmid-borne lacZ reporter cassettes, we show here how the global regulator H-NS affects the activity of the fimA promoter. In phase-locked reporter cassettes the activity of the fimA promoter was found to be enhanced in a hns-positive background, but only when the switch was in the ON orientation. Also, the number of fimbriae produced by a phase-locked ON strain was significantly higher in a hns-positive background. By means of competitive gel retardation and DNase I protection assays, H-NS binding to DNA segments adjacent to and within the phase switch region was demonstrated. |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/physiology; Base Sequence; Binding Sites/genetics; DNA Primers/genetics; DNA, Bacterial/genetics; DNA-Binding Proteins/physiology; Escherichia coli/genetics; Escherichia coli/physiology; Fimbriae Proteins; Fimbriae, Bacterial/genetics; Fimbriae, Bacterial/physiology; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genes, Switch; Molecular Sequence Data; Polymerase Chain Reaction; Promoter Regions, Genetic; Protein Binding |
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