PMID:9748461

Citation	Oliver, D, Norman, J and Sarker, S (1998) Regulation of Escherichia coli secA by cellular protein secretion proficiency requires an intact gene X signal sequence and an active translocon. J. Bacteriol. 180:5240-2
Abstract	secA is translationally regulated by the protein secretion proficiency state of the Escherichia coli cell. This regulation was explored by making signal sequence mutations in the gene upstream of secA, gene X, which promotes secA translational coupling. Gene X signal sequence mutants were constitutive for secA expression, while prlA alleles partially restored secA regulation. These results show that interaction of the pre-gene X protein with the translocon is required for proper secA regulation. Furthermore, gene X signal sequence mutations disrupted secA regulation only in the cis configuration. We propose that nascent pre-gene X protein interacts with the translocon during its secretion to constitute the secretion sensor.
Links	PubMed PMC107564
Keywords	Adenosine Triphosphatases/genetics; Bacterial Proteins/genetics; Bacterial Proteins/secretion; Chromosomes, Bacterial/genetics; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial/genetics; Membrane Transport Proteins; Mutation; Plasmids/genetics; Protein Sorting Signals/genetics
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