PMID:9740132
| Citation |
Shpanchenko, OV, Dontsova, OA, Bogdanov, AA and Nierhaus, KH (1998) Structure of 5S rRNA within the Escherichia coli ribosome: iodine-induced cleavage patterns of phosphorothioate derivatives. RNA 4:1154-64 |
|---|---|
| Abstract |
The protection patterns of 5S rRNA in solution, within the ribosomal 50S subunit, 70S ribosomes, and functional complexes, were assessed with the phosphorothioate method. About 20% of the analyzed positions (G9-G107) showed strong assembly defects: A phosphorothioate at one of these positions significantly impaired the incorporation of 5S rRNA into 50S particles. The reverse has also been observed: A phosphorothioate is preferred over a phosphate residue in the assembly process at a few positions. The results further demonstrate that 5S rRNA undergoes conformational changes during the assembly in the central protuberance of the 50S subunit and upon association with the small ribosomal subunit forming a 70S ribosome. In striking contrast, when the 70S ribosomes are once formed, the contact pattern of the 5S rRNA is the same in various functional states such as initiation-like complexes and pre- and posttranslocational states. |
| Links | |
| Keywords |
Base Sequence; Binding Sites/genetics; Escherichia coli/chemistry; Escherichia coli/genetics; Escherichia coli/metabolism; Iodine; Molecular Sequence Data; Nucleic Acid Conformation; RNA, Bacterial/chemistry; RNA, Bacterial/genetics; RNA, Bacterial/metabolism; RNA, Ribosomal, 5S/chemistry; RNA, Ribosomal, 5S/genetics; RNA, Ribosomal, 5S/metabolism; Ribosomes/chemistry; Ribosomes/metabolism; Thionucleotides/chemistry |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.