PMID:9729432
| Citation |
Bell, SJ, Chow, YC, Ho, JY and Forsdyke, DR (1998) Correlation of chi orientation with transcription indicates a fundamental relationship between recombination and transcription. Gene 216:285-92 |
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| Abstract |
Cross-over hot-spot instigator (Chi) sequences (5'-GCTGGTGG-3') are abundant, strand-specific, sequences, which locally increase recombination in Escherichia coli. Located within G-rich 'recombination islands', Chi orientations correlate with the orientations both of DNA replication and of transcription. Consistent with evidence from eukaryotic systems for a fundamental relationship between recombination and transcription, we find for E. coli Chi sequences, and for Haemophilus influenzae Chi-like sequences, that orientations correlate better with transcription than with replication. Complying with Szybalski's transcription direction rule, open reading frames in these prokaryotes have purine-rich mRNA-synonymous DNA strands. Hence, the G-richness of 'recombination islands' may reflect their correspondence with 'transcriptional islands' (genes). Comparison of a natural with the corresponding shuffled sequence, indicates a base order-dependent island unit of approx. 1kb. 1998 Elsevier Science B.V. |
| Links | |
| Keywords |
Base Composition; Base Sequence; Crossing Over, Genetic/genetics; DNA, Bacterial/chemistry; DNA, Bacterial/genetics; Escherichia coli/genetics; Haemophilus influenzae/genetics; Open Reading Frames/genetics; Recombination, Genetic/genetics; Transcription, Genetic/genetics |
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