PMID:9714555

Citation	Licher, T, Kellner, E and Lill, H (1998) The coupling region of F0F1 ATP synthase: binding of the hydrophilic loop of F0 subunit c to F1. FEBS Lett. 431:419-22
Abstract	The hydrophilic loop region of the CF0 c subunit has been expressed as a fusion with MalE in Escherichia coli. A cysteine was introduced at the C-terminus to allow fluorophore labeling of the fusion protein. After removal of the MalE moiety, the labeled peptide was used for binding studies with fluorophore-labeled CF1. At saturation, 1 mol peptide was bound per mol CF1. Binding was abolished after removal of subunit epsilon from CF1, and partially restored by addition of recombinant epsilon.
Links	PubMed
Keywords	ATP-Binding Cassette Transporters; Amino Acid Sequence; Carrier Proteins/metabolism; Escherichia coli/genetics; Escherichia coli Proteins; Maltose-Binding Proteins; Molecular Sequence Data; Monosaccharide Transport Proteins; Periplasmic Binding Proteins; Protein Binding; Proton-Translocating ATPases/metabolism; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Spectrometry, Fluorescence
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