PMID:9698562

Citation	Polyakov, A, Richter, C, Malhotra, A, Koulich, D, Borukhov, S and Darst, SA (1998) Visualization of the binding site for the transcript cleavage factor GreB on Escherichia coli RNA polymerase. J. Mol. Biol. 281:465-73
Abstract	The structure of Escherichia coli core RNA polymerase (RNAP) complexed with the transcript cleavage factor GreB was determined from electron micrographs of negatively stained, flattened helical crystals. A binding assay was developed to establish that GreB was incorporated into the RNA polymerase crystals with high occupancy through interactions between the globular C-terminal domain and the RNA polymerase. Comparison of the core RNAP:GreB structure with the previously determined structure of core RNAP located the GreB binding site on one face of the RNA polymerase, next to but not in the 25 A-diameter channel of RNA polymerase.
Links	PubMed Online version:10.1006/jmbi.1998.1958
Keywords	Binding Sites; Circular Dichroism; Crystallization; DNA-Directed RNA Polymerases/chemistry; Electrophoresis, Polyacrylamide Gel; Escherichia coli/chemistry; Escherichia coli/enzymology; Escherichia coli Proteins; Image Processing, Computer-Assisted/methods; Microscopy, Electron/methods; Transcription Factors/chemistry; Transcriptional Elongation Factors
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