PMID:9693099

Citation	Thiede, B, Urlaub, H, Neubauer, H, Grelle, G and Wittmann-Liebold, B (1998) Precise determination of RNA-protein contact sites in the 50 S ribosomal subunit of Escherichia coli. Biochem. J. 334 ( Pt 1):39-42
Abstract	RNA-protein cross-linked complexes were isolated and purified to obtain precise data about RNA-protein contact sites in the 50 S ribosomal subunit of Escherichia coli. N-terminal microsequencing and matrix-assisted laser desorption ionization MS were used to identify the cross-linking sites at the amino acid and nucleotide levels. In this manner the following contact sites of five ribosomal proteins with the 23 S rRNA were established: Lys-67 of L2 to U-1963, Tyr-35 of L4 to U-615, Lys-97 of L21 to U-546, Lys-49 of L23 to U-139 or C-140 and Lys-71 and Lys-74 of L27 to U-2334.
Links	PubMed PMC1219658
Keywords	Amino Acid Sequence; Bacterial Proteins/chemistry; Base Sequence; Binding Sites; Chromatography, High Pressure Liquid; Cross-Linking Reagents; Models, Molecular; Molecular Sequence Data; Nucleic Acid Conformation; Oligoribonucleotides/chemistry; Peptide Fragments/chemistry; RNA, Bacterial/chemistry; RNA, Ribosomal, 23S/chemistry; Ribosomal Proteins/chemistry; Ribosomes/chemistry; Ribosomes/ultrastructure; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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