PMID:9692897
| Citation |
Rieder, C, Eisenreich, W, O'Brien, J, Richter, G, Götze, E, Boyle, P, Blanchard, S, Bacher, A and Simon, H (1998) Rearrangement reactions in the biosynthesis of molybdopterin--an NMR study with multiply 13C/15N labelled precursors. Eur. J. Biochem. 255:24-36 |
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| Abstract |
The genes moaABC of Escherichia coli were ligated into the expression vector pNCO113. The resulting plasmid was transformed into a moeA mutant of E. coli. From cultures of the recombinant strain, a pteridine designated compound Z could be isolated at 5 mg/liter. Compound Z is a product of precursor Z, a biosynthetic precursor of molybdopterin. Cultures of the recombinant E. coli strain were supplied with [U-(13)C6]glucose, [U-(13)C5]ribulose 5-phosphate, or [7-(15)N,8-(13)C]guanine. The culture medium also contained a large excess of unlabeled glucose. Compound Z as well as nucleosides obtained by hydrolysis of RNA were isolated from the bacterial cultures, and their heavy isotope distribution was investigated by one-dimensional and two-dimensional NMR spectroscopy. The labelling patterns of compound Z show that the carbon atoms of a pentose or pentulose are diverted to the ring atoms C6 and C7 and to the side chain atoms C2', C3' and C4' of compound Z. Carbon atom C1' of compound Z is derived from carbon atom C8 of a guanine derivative. The remodeling of the carbon skeleton of the pentose and purine moieties proceed via intramolecular rearrangement reactions. |
| Links | |
| Keywords |
Carbon Isotopes; Coenzymes/biosynthesis; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Glucose/metabolism; Guanine/analogs & derivatives; Guanine/metabolism; Isotope Labeling; Metalloproteins/metabolism; Models, Chemical; Molybdenum/metabolism; Mutation; Nitrogen Isotopes; Nuclear Magnetic Resonance, Biomolecular; Pteridines/metabolism; Ribulosephosphates/metabolism; Sulfurtransferases/genetics |
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