PMID:9683493
| Citation |
Trusca, D, Scott, S, Thompson, C and Bramhill, D (1998) Bacterial SOS checkpoint protein SulA inhibits polymerization of purified FtsZ cell division protein. J. Bacteriol. 180:3946-53 |
|---|---|
| Abstract |
Cell division of Escherichia coli is inhibited when the SulA protein is induced in response to DNA damage as part of the SOS checkpoint control system. The SulA protein interacts with the tubulin-like FtsZ division protein. We investigated the effects of purified SulA upon FtsZ. SulA protein inhibits the polymerization and the GTPase activity of FtsZ, while point mutant SulA proteins show little effect on either of these FtsZ activities. SulA did not inhibit the polymerization of purified FtsZ2 mutant protein, which was originally isolated as insensitive to SulA. These studies define polymerization assays for FtsZ which respond to an authentic cellular regulator. The observations presented here support the notion that polymerization of FtsZ is central to its cellular role and that direct, reversible inhibition of FtsZ polymerization by SulA may account for division inhibition. |
| Links | |
| Keywords |
Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Bacterial Proteins/ultrastructure; Cell Division; Chromatography, Affinity; Cytoskeletal Proteins; DNA Damage; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; GTP Phosphohydrolases/metabolism; Isopropyl Thiogalactoside/pharmacology; Kinetics; Macromolecular Substances; Microscopy, Electron; Mutagenesis, Site-Directed; Recombinant Fusion Proteins/isolation & purification; Recombinant Fusion Proteins/metabolism; Recombinant Fusion Proteins/ultrastructure; SOS Response (Genetics) |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.