PMID:9679295
| Citation |
Rajagopal, P, Jones, BE and Klevit, RE (1998) Solvent exchange rates of side-chain amide protons in proteins. J. Biomol. NMR 11:205-12 |
|---|---|
| Abstract |
Solvent exchange rates and temperature coefficients for Asn/Gln side-chain amide protons have been measured in Escherichia coli HPr. The protons of the eight side-chain amide groups (two Asn and six Gln) exhibit varying exchange rates which are slower than some of the fast exchanging backbone amide protons. Differences in exchange rates of the E and Z protons of the same side-chain amide group are obtained by measuring exchange rates at pH values > 8. An NOE between a side-chain amide proton and a bound water molecule was also observed. |
| Links | |
| Keywords |
Bacterial Proteins/chemistry; Escherichia coli; Phosphoenolpyruvate Sugar Phosphotransferase System/chemistry; Protons |
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