PMID:9671026
| Citation |
Havre, PA and Kmiec, EB (1998) RecA-mediated joint molecule formation between O-methylated RNA/DNA hairpins and single-stranded targets. Mol. Gen. Genet. 258:580-6 |
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| Abstract |
Chimeric oligonucleotides consisting of one DNA strand paired with an O-methylated RNA strand interrupted by six DNA residues have been used in gene targeting experiments. Here we demonstrate that these hairpins can form a heteroduplex (or joint molecule) with single-stranded DNA targets in a reaction mediated by the E. coli RecA protein. One end of the double hairpin may unwind to form a 14-base-RecA filament which initiates the reaction. Chimeric oligonucleotides containing only O-methylated RNA residues on one strand or truncated hairpins lacking this 14-base segment did not participate in RecA-driven heteroduplex formation under these reaction conditions. The results presented here represent a first step in studying one facet of a strategy which uses O-methylated RNA residues as participants in homologous pairing events. |
| Links | |
| Keywords |
Base Sequence; DNA, Single-Stranded/chemistry; Methylation; Molecular Sequence Data; Nucleic Acid Conformation; Oligonucleotides/chemical synthesis; Oligonucleotides/chemistry; Protein Binding; RNA/chemistry; Rec A Recombinases/chemistry |
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