PMID:9632265
| Citation |
Afflerbach, H, Schröder, O and Wagner, R (1998) Effects of the Escherichia coli DNA-binding protein H-NS on rRNA synthesis in vivo. Mol. Microbiol. 28:641-53 |
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| Abstract |
The Escherichia coli DNA-binding protein H-NS is known to interact specifically with the upstream region of ribosomal RNA transcription units, where it causes transcriptional repression in vitro. Here, we present results demonstrating the effect of H-NS on rRNA transcription in vivo. rRNA synthesis rates were compared in cells that differ in the expression of functional H-NS or FIS molecules. We could show that in the absence of H-NS derepression of rRNA synthesis occurs at low growth rates. During the cell cycle H-NS is responsible for the rapid shut-off of rRNA synthesis at the end of the exponential phase. As it is known for FIS-dependent activation, the inhibitory function of H-NS is specific for P1, the first of the tandem rRNA promoters. The effect of H-NS on rRNA synthesis was further assessed under stress conditions. While under osmotic upshift the reduction in rRNA synthesis is clearly H-NS-dependent, no such influence could be detected at cold shock. Determination of the cellular ppGpp concentrations revealed that H-NS does not mediate its function via alterations in the synthesis of the global effector ppGpp. |
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| Keywords |
Bacterial Proteins; Carrier Proteins/genetics; Carrier Proteins/metabolism; Chloramphenicol O-Acetyltransferase/biosynthesis; Chloramphenicol O-Acetyltransferase/genetics; Cold Temperature; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli Proteins; Factor For Inversion Stimulation Protein; Gene Expression Regulation, Bacterial; Guanosine Tetraphosphate/metabolism; Integration Host Factors; Osmotic Pressure; Promoter Regions, Genetic; RNA, Bacterial/biosynthesis; RNA, Ribosomal/biosynthesis; Repressor Proteins; Transcription, Genetic; rRNA Operon |
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