PMID:9627402
| Citation |
Mikola, MR, Widman, MT and Worden, RM (1998) In situ mutagenesis and chemotactic selection of microorganisms in a diffusion gradient chamber. Appl. Biochem. Biotechnol. 70-72:905-18 |
|---|---|
| Abstract |
A new method has been developed to rapidly generate and select microbial strains having increased resistance to an inhibitory compound. The method combines in situ mutagenesis with use of a continuous gradient of the inhibitor to sort cells according to their resistance levels. Microbial chemotaxis is induced to accelerate the selection process. The method was used to develop a strain of E. coli having a feedback-resistant DAHP synthase enzyme. An unsteady-state mathematical model of the process has been developed. The model, that can reproduce key trends observed experimentally, was used to explore the effects of chemotaxis on the efficiency of the selection process. |
| Links | |
| Keywords |
3-Deoxy-7-Phosphoheptulonate Synthase/analysis; Chemotaxis; Diffusion Chambers, Culture; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/radiation effects; Glucose/chemistry; Models, Theoretical; Mutagenesis, Site-Directed; Ultraviolet Rays |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.