PMID:9613586
| Citation |
Sirko, A, Wegleńska, A and Hulanicka, D (1998) Integration host factor positively regulates cycJIH transcription. Mol. Gen. Genet. 258:174-7 |
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| Abstract |
We report the identification of integration host factor (IHF) as an additional element involved in the regulation of the cysJIH promoter of Salmonella typhimurium and Escherichia coli. An IHF-binding site was located in the regulatory region of the cysJIH promoter by using two methods, protection against DNase I digestion and hydroxyl radical cleavage. The positive influence of IHF on in vitro run-off transcription from the cysJIH promoter is shown. The in vivo observations suggest that IHF is necessary for full expression of cysJIH in stationary phase but not during exponential growth. |
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| Keywords |
Bacterial Proteins/pharmacology; Base Sequence; Binding Sites; Cysteine/biosynthesis; Escherichia coli/genetics; Gene Expression Regulation; Integration Host Factors; Molecular Sequence Data; Mutation; Promoter Regions, Genetic; Salmonella typhimurium/genetics |
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