PMID:9603904
| Citation |
Cheng, ZF, Zuo, Y, Li, Z, Rudd, KE and Deutscher, MP (1998) The vacB gene required for virulence in Shigella flexneri and Escherichia coli encodes the exoribonuclease RNase R. J. Biol. Chem. 273:14077-80 |
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| Abstract |
vacB, a gene previously shown to be required for expression of virulence in Shigella and enteroinvasive Escherichia coli, has been found to encode the 3'-5' exoribonuclease, RNase R. Thus, cloning of E. coli vacB led to overexpression of RNase R activity, and partial deletion or interruption of the cloned gene abolished this overexpression. Interruption of the chromosomal copy of vacB eliminated endogenous RNase R activity; however, the absence of RNase R by itself had no effect on cell growth. In contrast, cells lacking both RNase R and polynucleotide phosphorylase were found to be inviable. These data indicate that RNase R participates in an essential cell function in addition to its role in virulence. The identification of the vacB gene product as RNase R should aid in understanding how the virulence phenotype in enterobacteria is expressed and regulated. On the basis of this information we propose that vacB be renamed rnr. |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/physiology; Cloning, Molecular; Endoribonucleases/genetics; Endoribonucleases/physiology; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins; Exoribonucleases; Gene Expression Regulation, Bacterial/genetics; Genes, Bacterial/genetics; Phenotype; Poly A/metabolism; Polyribonucleotide Nucleotidyltransferase/physiology; Restriction Mapping; Shigella flexneri/enzymology; Shigella flexneri/genetics; Transduction, Genetic/genetics; Virulence/genetics |
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