PMID:9593299
| Citation |
Suzuki, E, Kondo, T, Makise, M, Mima, S, Sakamoto, K, Tsuchiya, T and Mizushima, T (1998) Alteration in the contents of unsaturated fatty acids in dnaA mutants of Escherichia coli. Mol. Microbiol. 28:95-102 |
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| Abstract |
DnaA protein, the initiator of chromosomal DNA replication in Escherichia coli, has a high affinity for acidic phospholipids containing unsaturated fatty acids. We have examined here the fatty acid composition of phospholipids in dnaA mutants. A temperature-sensitive dnaA46 mutant showed a lower level of unsaturation of fatty acids (ratio of unsaturated to saturated fatty acids) at 42 degrees C (non-permissive temperature) and at 37 degrees C (semi-permissive temperature), but not at 28 degrees C (permissive temperature), compared with the wild-type strain. Plasmid complementation analysis revealed that the dnaA46 mutation is responsible for the phenotype. Other temperature-sensitive dnaA mutants showed similar results. On the other hand, a cold-sensitive dnaAcos mutant, in which over-initiation of DNA replication occurs at low temperature (28 degrees C), showed a higher level of unsaturation of fatty acids at 28 degrees C. Based on these observations, we discuss the role of phospholipids in the regulation of the activity of DnaA protein. |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Cell Division; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/chemistry; Escherichia coli/genetics; Escherichia coli/metabolism; Fatty Acids, Unsaturated/analysis; Fatty Acids, Unsaturated/metabolism; Genetic Complementation Test; Mutation; Phospholipids/analysis; Plasmids/genetics; Replication Origin/genetics; Temperature |
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