PMID:9573153
| Citation |
Hu, ST, Wang, HC, Lei, GS and Wang, SH (1998) Negative regulation of IS2 transposition by the cyclic AMP (cAMP)-cAMP receptor protein complex. J. Bacteriol. 180:2682-8 |
|---|---|
| Abstract |
Three sequences similar to that of the consensus binding sequence of the cyclic AMP (cAMP)-cAMP receptor protein (CRP) complex were found in the major IS2 promoter region. Experiments were performed to determine whether the cAMP-CRP complex plays a role in the regulation of IS2 transposition. In the gel retardation assay, the cAMP-CRP complex was found to be able to bind the major IS2 promoter. A DNA footprinting assay confirmed that the cAMP-CRP complex binds to the sequences mentioned above. With an IS2 promoter-luciferase gene fusion construct, the cAMP-CRP complex was shown to inhibit transcription from the major IS2 promoter. IS2 was found to transpose at a frequency approximately 200-fold higher in an Escherichia coli host defective for CRP or adenyl cyclase than in a wild-type host. These results suggest that the cAMP-CRP complex is a negative regulator of IS2 transposition. |
| Links | |
| Keywords |
Bacterial Proteins/metabolism; Base Sequence; Carrier Proteins; Cyclic AMP Receptor Protein/metabolism; DNA Transposable Elements/genetics; DNA, Bacterial/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Promoter Regions, Genetic/genetics; Sequence Alignment; Transcription, Genetic |
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