PMID:9568583
| Citation |
Grzesiuk, E and Janion, C (1998) Mutation frequency decline in MMS-treated Escherichia coli K-12 mutS strains. Mutagenesis 13:127-32 |
|---|---|
| Abstract |
It has been shown that the decline in mutant frequency (MFD) (argE3(ochre)-->Arg+) which occurs in MMS-treated and then transiently starved AB1157 Escherichia coli K-12 cells concerns revertants which arose by supL suppressor formation in a process which is umuDC dependent. Here we have examined whether MMS-induced Arg+ revertants are susceptible to decline when bacteria are deficient in mismatch repair. We show that there is an absence of MFD in MMS-treated M1 (mutS) and in EC2416 (mutS delta umuDC) cells defective in mismatch repair which is associated with a change in the spectrum of MMS-induced mutations formed. In contrast to AB1157, transformation of M1 (mutS) bacteria with plasmids harbouring various combinations of umuD(D')C genes does not enhance the level of MMS-induced mutations but may influence the proportion of supL mutations. These supL mutations show MFD. Repair processes under MFD conditions were confirmed by analysis of plasmid DNA isolated from MMS-treated bacteria at different stages of their starvation and digestion with Fpg protein. |
| Links | |
| Keywords |
Adenosine Triphosphatases; Bacterial Proteins/biosynthesis; Bacterial Proteins/drug effects; Bacterial Proteins/genetics; DNA Repair/drug effects; DNA-Binding Proteins; DNA-Directed DNA Polymerase; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli Proteins; Methyl Methanesulfonate/toxicity; MutS DNA Mismatch-Binding Protein; Mutagenesis/drug effects; Mutagens; Mutation/drug effects |
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