PMID:9555915
| Citation |
Yamashino, T, Isomura, M, Ueguchi, C and Mizuno, T (1998) The yhhP gene encoding a small ubiquitous protein is fundamental for normal cell growth of Escherichia coli. J. Bacteriol. 180:2257-61 |
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| Abstract |
H-NS is a major constituent of the Escherichia coli nucleoid, whereas sigmaS is a stress-induced sigma factor. An hns null mutation affects the cellular content of sigmaS in such a way that a remarkable accumulation of sigmaS is observed in the logarithmic growth phase, which results in enhanced expression of a number of sigmaS-dependent genes, including the katE gene. We isolated an extragenic mutation that affects the expression of the katE-lacZ fusion gene in the deltahns background. The relevant gene was identified as yhhP, which encodes a small polypeptide of 81 amino acids. Lesion of this gene seemed to affect the stability of sigmaS. A deletion analysis of yhhP revealed that this small protein plays a fundamental role in the general physiology of E. coli. The yhhP-deficient cell is not capable of growing in standard laboratory rich medium (i.e., Luria broth), resulting in the formation of filamentous cells. Homologs of this intriguing protein occur in a wide variety of bacterial species, including archaeal species. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/biosynthesis; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/physiology; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Kinetics; Molecular Sequence Data; Mutagenesis; Recombinant Fusion Proteins/biosynthesis; Restriction Mapping; Sequence Alignment; Sequence Homology, Amino Acid; Sigma Factor/biosynthesis |
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