PMID:9548457
| Citation |
Youn, BS, Zhang, SM, Lee, EK, Park, DH, Broxmeyer, HE, Murphy, PM, Locati, M, Pease, JE, Kim, KK, Antol, K and Kwon, BS (1997) Molecular cloning of leukotactin-1: a novel human beta-chemokine, a chemoattractant for neutrophils, monocytes, and lymphocytes, and a potent agonist at CC chemokine receptors 1 and 3. J. Immunol. 159:5201-5 |
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| Abstract |
A new member of human beta-chemokine cDNA was isolated and named leukotactin-1 (Lkn-1). Lkn-1, along with murine macrophage inflammatory protein-related protein-1 and -2, defines a subgroup of beta-chemokines based on two conserved cysteines in addition to the four others conserved in all beta-chemokines. The putative mature Lkn-1 is composed of 92 amino acids with a calculated m.w. of 10,162. The Lkn-1 gene was mapped to human chromosome 17, region q12. Recombinant Lkn-1 was a potent chemoattractant for neutrophils, monocytes, and lymphocytes and induced calcium flux in these cells. Lkn-1 specifically induced calcium flux in CCR1- and CCR3-expressing HOS cell lines. Lkn-1 suppressed colony formation by human granulocyte-macrophage, erythroid, and multipotential progenitor cells stimulated by combinations of growth factors. Hence, we have isolated and characterized a human C6 beta-chemokine that is a potent agonist at CCR1 and CCR3 and shows broad biologic activities, including leukocyte chemoattraction. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; Calcium/metabolism; Chemokines, CC/genetics; Chemokines, CC/immunology; Chemotaxis; Chromosome Mapping; Chromosomes, Human, Pair 17; Cloning, Molecular; Humans; Leukocytes/immunology; Lymphocytes/immunology; Lymphocytes/metabolism; Molecular Sequence Data; Monocytes/immunology; Monocytes/metabolism; Neutrophils/immunology; Neutrophils/metabolism; Receptors, Chemokine/antagonists & inhibitors; Recombinant Proteins/genetics; Time Factors |
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