PMID:9547274
| Citation |
Firpo, MA and Dahlberg, AE (1998) The importance of base pairing in the penultimate stem of Escherichia coli 16S rRNA for ribosomal subunit association. Nucleic Acids Res. 26:2156-60 |
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| Abstract |
The influence of base pairing in the penultimate stem of Escherichia coli 16S rRNA (defined as nt 1409-1491) on ribosome function has been addressed by the construction of mutations in this region of rRNA. Two sets of mutations were made on either side of a structurally conserved region in the penultimate stem that disrupted base pairing, while a third set of mutations replaced the wild-type sequence with other base pair combinations. The effects of these mutations were analyzed in vivo and in vitro . The mutations that disrupted base pairing caused significant increases in cell doubling times as well as a severe subunit association defect and a modest increase in frame shifting and stop codon read-through. Restoration of base pairing restored wild-type growth rates, decoding and subunit association, indicating that base pairing in this region is essential for proper ribosome function. |
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| Keywords |
Base Sequence; Escherichia coli; Molecular Sequence Data; Mutagenesis, Site-Directed; Nucleic Acid Conformation; Polyribosomes/metabolism; Protein Biosynthesis; RNA, Bacterial/genetics; RNA, Bacterial/metabolism; RNA, Double-Stranded/genetics; RNA, Double-Stranded/metabolism; RNA, Ribosomal, 16S/genetics; RNA, Ribosomal, 16S/metabolism; Ribosomes/metabolism |
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