PMID:9535658
| Citation |
Green, R, Switzer, C and Noller, HF (1998) Ribosome-catalyzed peptide-bond formation with an A-site substrate covalently linked to 23S ribosomal RNA. Science 280:286-9 |
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| Abstract |
In the ribosome, the aminoacyl-transfer RNA (tRNA) analog 4-thio-dT-p-C-p-puromycin crosslinks photochemically with G2553 of 23S ribosomal RNA (rRNA). This covalently linked substrate reacts with a peptidyl-tRNA analog to form a peptide bond in a peptidyl transferase-catalyzed reaction. This result places the conserved 2555 loop of 23S rRNA at the peptidyl transferase A site and suggests that peptide bond formation can occur uncoupled from movement of the A-site tRNA. Crosslink formation depends on occupancy of the P site by a tRNA carrying an intact CCA acceptor end, indicating that peptidyl-tRNA, directly or indirectly, helps to create the peptidyl transferase A site. |
| Links | |
| Keywords |
Anti-Bacterial Agents/pharmacology; Binding Sites; Catalysis; Enzyme Inhibitors/pharmacology; Escherichia coli; Nucleic Acid Conformation; Peptidyl Transferases/antagonists & inhibitors; Peptidyl Transferases/metabolism; Puromycin/analogs & derivatives; Puromycin/chemical synthesis; Puromycin/chemistry; Puromycin/metabolism; RNA, Bacterial/chemistry; RNA, Bacterial/metabolism; RNA, Ribosomal, 23S/chemistry; RNA, Ribosomal, 23S/metabolism; RNA, Transfer, Amino Acyl/chemistry; RNA, Transfer, Amino Acyl/metabolism; RNA, Transfer, Phe/chemistry; RNA, Transfer, Phe/genetics; RNA, Transfer, Phe/metabolism; Ribosomes/metabolism |
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