PMID:9507009
| Citation |
Sukhodolets, MV and Jin, DJ (1998) RapA, a novel RNA polymerase-associated protein, is a bacterial homolog of SWI2/SNF2. J. Biol. Chem. 273:7018-23 |
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| Abstract |
We have identified a novel Escherichia coli RNA polymerase (RNAP)-associated protein, an ATPase named RapA. Almost all of this 110-kDa protein in the cell copurifies with RNAP holoenzyme as a 1:1 complex. Purified to homogeneity, RapA also forms a stable complex with RNAP, as if it were a subunit of RNAP. The ATPase activity of RapA is stimulated by binding to RNAP, and thus, RapA and RNAP interact physically as well as functionally. Interestingly, RapA is a homolog of the SWI/SNF family of eukaryotic proteins whose members are involved in transcription activation, nucleosome remodeling, and DNA repair. |
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| Keywords |
Adenosine Triphosphatases/metabolism; Chromatography, Ion Exchange; DNA-Binding Proteins/chemistry; DNA-Binding Proteins/isolation & purification; DNA-Binding Proteins/metabolism; DNA-Directed RNA Polymerases/metabolism; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Escherichia coli/metabolism; Nuclear Proteins; Transcription Factors/chemistry; Transcription Factors/isolation & purification; Transcription Factors/metabolism |
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