PMID:9501232
| Citation |
Bessarab, DA, Kaberdin, VR, Wei, CL, Liou, GG and Lin-Chao, S (1998) RNA components of Escherichia coli degradosome: evidence for rRNA decay. Proc. Natl. Acad. Sci. U.S.A. 95:3157-61 |
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| Abstract |
Recently, we found that a multicomponent ribonucleolytic degradosome complex formed around RNase E, a key mRNA-degrading and 9S RNA-processing enzyme, contains RNA in addition to its protein components. Herein we show that the RNA found in the degradosome consists primarily of rRNA fragments that have a range of distinctive sizes. We further show that rRNA degradation is carried out in the degradosome by RNase E cleavage of A+U-rich single-stranded regions of mature 16S and 23S rRNAs. The 5S rRNA, which is known to be generated by RNase E processing of the 9S precursor, was also identified in the degradosome, but tRNAs, which are not cleaved by RNase E in vitro, were absent. Our results, which provide evidence that decay of mature rRNAs occurs in growing Escherichia coli cells in the RNA degradosome, implicate RNase E in degradosome-mediated decay. |
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| Keywords |
Chromatography, Affinity; Endoribonucleases/immunology; Endoribonucleases/isolation & purification; Endoribonucleases/metabolism; Escherichia coli/enzymology; Multienzyme Complexes/immunology; Multienzyme Complexes/isolation & purification; Multienzyme Complexes/metabolism; Peptides; Polyribonucleotide Nucleotidyltransferase/immunology; Polyribonucleotide Nucleotidyltransferase/isolation & purification; Polyribonucleotide Nucleotidyltransferase/metabolism; RNA Helicases; RNA Precursors/metabolism; RNA Processing, Post-Transcriptional; RNA, Bacterial/metabolism; RNA, Ribosomal/metabolism; RNA, Ribosomal, 16S/metabolism; RNA, Ribosomal, 23S/metabolism; RNA, Ribosomal, 5S/metabolism; RNA, Transfer/metabolism; Substrate Specificity |
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