PMID:9501185
| Citation |
Mukherjee, A, Cao, C and Lutkenhaus, J (1998) Inhibition of FtsZ polymerization by SulA, an inhibitor of septation in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 95:2885-90 |
|---|---|
| Abstract |
The bacterial cell division protein FtsZ assembles into the cytokinetic Z ring that directs cytokinesis in prokaryotes. In Escherichia coli the formation of the Z ring is prevented by induction of the cell division inhibitor SulA (SfiA), a component of the SOS response. Here we show that a MalE-SulA fusion that retains this inhibitory function in vivo inhibits the GTPase activity and polymerization of FtsZ in vitro. MalE-SulA10, which does not block Z ring formation in vivo, is unable to inhibit the GTPase activity and polymerization in vitro. Furthermore, FtsZ114, which is refractory to SulA in vivo, is not inhibited by MalE-SulA. These results indicate that SulA blocks Z ring formation by blocking FtsZ polymerization. |
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| Keywords |
ATP-Binding Cassette Transporters; Bacterial Proteins/antagonists & inhibitors; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacterial Proteins/pharmacology; Carrier Proteins/genetics; Carrier Proteins/pharmacology; Cell Division; Cytoskeletal Proteins; Escherichia coli/growth & development; Escherichia coli Proteins; GTP Phosphohydrolases/antagonists & inhibitors; GTP Phosphohydrolases/metabolism; GTP-Binding Proteins/antagonists & inhibitors; GTP-Binding Proteins/metabolism; Maltose-Binding Proteins; Monosaccharide Transport Proteins; Periplasmic Binding Proteins; Protein Binding/drug effects; Recombinant Fusion Proteins/pharmacology |
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