PMID:9498571
| Citation |
Ogawa, W, Kayahara, T, Tsuda, M, Mizushima, T and Tsuchiya, T (1997) Isolation and characterization of an Escherichia coli mutant lacking the major serine transporter, and cloning of a serine transporter gene. J. Biochem. 122:1241-5 |
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| Abstract |
L-Serine as well as L-valine inhibits the growth of Escherichia coli cells, and L-isoleucine releases this growth inhibition. We isolated an E. coli mutant (designated as WAT9) that was able to grow on lactate (or glucose) as a carbon source even in the presence of L-serine, the parent not being able to. Cells of WAT9 were not able to grow on L-serine as a carbon source even if L-isoleucine was present in the culture medium, while the parental cells grew. This mutant was shown to lack the principal L-serine transporter in E. coli, the Na+/ serine symporter. This mutant is useful for analysis of the role(s) of the Na+/serine symporter in cell physiology and as a host for the cloning of L-serine transporter gene(s). In fact, we cloned a gene encoding a serine transporter from chromosomal DNA of E. coli using WAT9 as the host. The gene enabled the mutant cells to grow on L-serine. Transport activity for L-serine was restored in the mutant cells harboring a plasmid carrying the gene. We partially sequenced the gene and found that it was the tdcC gene. We showed that TdcC is an H+/serine symporter. |
| Links | |
| Keywords |
Amino Acid Transport Systems; Amino Acid Transport Systems, Neutral; Biological Transport; Carrier Proteins/genetics; Cloning, Molecular; DNA, Bacterial/genetics; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli Proteins; Membrane Transport Proteins/genetics; Membrane Transport Proteins/metabolism; Mutation; Serine/metabolism; Serine/pharmacology |
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