PMID:9490010
| Citation |
Starkova, NN, Koroleva, EP, Rumsh, LD, Ginodman, LM and Rotanova, TV (1998) Mutations in the proteolytic domain of Escherichia coli protease Lon impair the ATPase activity of the enzyme. FEBS Lett. 422:218-20 |
|---|---|
| Abstract |
Conserved residues of the proteolytic domain of Escherichia coli protease Lon, putative members of the classic catalytic triad (H665, H667, D676, and D743) were identified by comparison of amino acid sequences of Lon proteases. Mutant enzymes containing substitutions D676N, D743N, H665Y, and H667Y were obtained by site-directed mutagenesis. The mutant D743N retained the adenosine triphosphate (ATP)-dependent proteolytic activity, thereby indicating that D743 does not belong to the catalytic site. Simultaneously, the mutants D676N, H665Y, and H667Y lost the capacity for hydrolysis of protein substrates. The ATPase activity of these three mutants was decreased by more than an order of magnitude, which suggests a close spatial location of the ATPase and proteolytic active sites and their tight interaction in the process of protein degradation. |
| Links | |
| Keywords |
ATP-Dependent Proteases; Adenosine Triphosphatases/chemistry; Adenosine Triphosphatases/metabolism; Amino Acid Sequence; Binding Sites; Brain/enzymology; Escherichia coli/enzymology; Escherichia coli Proteins; Heat-Shock Proteins/chemistry; Heat-Shock Proteins/metabolism; Humans; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Protease La; Recombinant Proteins/chemistry; Recombinant Proteins/metabolism; Sequence Alignment; Sequence Homology, Amino Acid; Serine Endopeptidases/chemistry; Serine Endopeptidases/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.