PMID:9466941

Citation	Navaratnam, N, Fujino, T, Bayliss, J, Jarmuz, A, How, A, Richardson, N, Somasekaram, A, Bhattacharya, S, Carter, C and Scott, J (1998) Escherichia coli cytidine deaminase provides a molecular model for ApoB RNA editing and a mechanism for RNA substrate recognition. J. Mol. Biol. 275:695-714
Abstract	ApoB RNA-editing enzyme (APOBEC-1) is a cytidine deaminase. Molecular modeling and mutagenesis show that APOBEC-1 is related in quaternary and tertiary structure to Escherichia coli cytidine deaminase (ECCDA). Both enzymes form a homodimer with composite active sites constructed with contributions from each monomer. Significant gaps are present in the APOBEC-1 sequence, compared to ECCDA. The combined mass of the gaps (10 kDa) matches that for the minimal RNA substrate. Their location in ECCDA suggests how APOBEC-1 can be reshaped to accommodate an RNA substrate. In this model, the asymmetrical binding to one active site of a downstream U (equivalent to the deamination product) helps target the other active site for deamination of the upstream C substrate.
Links	PubMed Online version:10.1006/jmbi.1997.1506
Keywords	Amino Acid Sequence; Apolipoproteins B/genetics; Apolipoproteins B/metabolism; Binding Sites; Cytidine Deaminase/genetics; Cytidine Deaminase/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Models, Molecular; Molecular Sequence Data; RNA Editing; RNA, Bacterial/metabolism; Sequence Alignment; Sequence Homology, Amino Acid; Substrate Specificity
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

Useful Materials and Methods

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Annotations

<annotationlinks/>

EcoliWiki Links

Add links to pages that link here (e.g. gene, product, method pages)

References

See Help:References for how to manage references in EcoliWiki.